An ultra-sensitive dopamine measurement platform based on molecularly imprinted polymer-carbon hybrid nanomaterials for in vitro use

In the present study, we designed an ultrasensitive sensing platform for evaluation of physiologically relevant values basal dopamine (DA) in a culture medium as complex biological environment. The proposed was fabricated via integration molecular imprinting technology with carbon hybrid nanomaterials. Carbon nanofibers (CNFs) were grown by using plasma-enhanced chemical vapor deposition (PECVD) on tetrahedral amorphous (ta-C) thin films silicon wafers. prepared ta-C/CNFs platforms electrochemically coated DA-imprinted polypyrrole molecularly imprinted polymer (MIP) or "artificial receptors". three-dimensional MIP receptors able to determine trace DA phosphate-buffered saline solution (PBS) pH 7.4 (LOD = 5.43 nM) well absolute media such DMEM/F-12 39 nM), supplemented 15% horse serum and 2.5% fetal bovine 53.26 F-12 K cell 62.57 highly sensitivity free interference other coexisting biomolecules compounds. As all fabrication steps composite electrode are compatible common microsystem processes, results pave way integrating these ultra-sensitive electrodes microelectrode arrays (MEA) used human dopaminergic neurons studies vitro enable continuous measurement concentration real-time instance organoid studies.